Ultimi articoli e pubblicazioni

Portfolio brevetti

Brevetti posseduti dai partners del programma Superpig:

  1. Galli C & Lazzari G. (1999). International application No PCT/EP99/02624 (No. 99922106.2). Source of nuclei for nuclear transfer.
  2. Lazzari G & Galli C. (2004). National application No 4649 PTIP Use of gametes and preimplantation embryos for toxicity screening of biologically active compounds.
  3. Maurizio Botta, Federico Corelli, Elena Petricci, Marco Radi, Giovanni Maga, Josè A. Este’, Antonello Mai “6-Vinyl pyrimidine and pyrimidinone derivatives and the use thereof” WO 2007/043094 A2
  4. Maurizio Botta, Marco Radi, Fabrizio Manetti, Giorgia Botta, Giovanni Maga, Jurgen Borlak “Derivati 1,3,4-tiadiazolici, metodi di preparazione e uso di essi” RM2007A000222.
  5. Schenone Silvia, Bondavalli Francesco, Bruno Olga, Botta Maurizio, Manetti Fabrizio, Radi Marco, Santucci Alessandra, Maga Giovanni, Bologna Mauro, Angelucci Adriano, Santucci Annalisa, Spreafico Adriano, Carraro Fabio “ New 4-substituted derivatives of pyrazolo [3,4-d]pyrimidine and pyrrolo[2,3-d]pyrimidine and uses thereof” Provisional priority application (US provisional n. 60/972,314).
  6. Brevetto n° MI97A001972. “Animali transgenici per lo studio di agenti tossici chimici, fisici o biologici”. Depositato il 28.8.1997. Esteso successivamente all’estero.
  7. Brevetto n° TO2002A00729. “Cellule di mammifero non umano geneticamente modificate, procedimento per la loro produzione e utilizzo in test di tossicità”. Depositato il 14.8.2002. Esteso successivamente all’estero.
  8. European Patent Application No. 07801625.0 in the name of Cesare Peschle and Gianluigi Condorelli Titolo Brevetto: MicroRNA 133 and 1 in cardiac hypertrophy and failure. International Application Number: PCT/EP2007/007131- anno 2006. International Application Number: PCT/EP2007/007131.

Pubblicazioni

Referenze Bibliografiche del programma “Superpig”

  1. Araki, K., Araki, M. & Yamamura, K. (1997).Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res 25, 868-872
  2. Benarroch, D., B. Selisko, G. A. Locatelli, G. Maga, J. L. Romette, and B. Canard. (2004). The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core. Virology 328:208-18.
  3. Benigni A, Perico N, Dadan J, Gabanelli M, Galbusera M, Boccardo P, Mennini T, Remuzzi G. (1990)Functional implications of decreased renal cortical atrial natriuretic peptide binding in experimental diabetes. Circ Res.; 66: 1453-1460.
  4. Benigni A, Tomasoni S, Turka LA, Longaretti L, Zentilin L, Mister M, Pezzotta A, Azzollini N, Noris M, Conti S, Abbate M, Giacca M, Remuzzi G. (2006)Adeno-associated virus-mediatedCTLA4Ig gene transfer protects MHC-mismatched renal allografts from chronic rejection. J Am Soc Nephrol.; 17: 1665-1672.
  5. Berlivet, S., V. Guiraud, M. Houlard, and M. Gerard. (2007). pHYPER, a shRNA vector for high-efficiency RNA interference in embryonic stem cells. BioTechniques 42:738, 740-3.
  6. Biamonti, G., Paixao, S., Montecucco, A., Peverali, F. A., Riva, S., and Falaschi, A. (2003). Is DNA sequence sufficient to specify DNA replication origins in metazoan cells? Chromosome Res 11, 403-412.
  7. Botta. (2008). Pharmacophore modeling and molecular docking led to the discovery of inhibitors of human immunodeficiency virus-1 replication targeting the human cellular aspartic acid-glutamic acid-alanine-aspartic acid box polypeptide 3. J Med Chem 51:6635-8.
  8. Bottiroli G. & Croce A.C. (2004). Autofluorescence spectroscopy of cells and tissues as a tool for biomedical diagnosis. Lasers and Current Optical techniques in Biology. Comprehensive series in Photochemical and Photobiological Sciences. RSC. vol. 4, 189-210.
  9. Bottiroli G. & Croce A.C. (2008). Autofluorescence spectroscopy for diagnosis: from cells to tissues (review). Energy for Healt, 2: 22-31.
  10. Bottiroli G. & Croce Ac. (2009). Optical biopsy: a promising approach for real-time liver steatosis grading (Editorial). Liver Int., 29: 321-322.
  11. Bottiroli G., Croce A.C., Locatelli D., Marchesini R., Pignoli E., Tomatis S., Cuzzoni C., Di Palma S., Dal Fante M., Spinelli P. (1995). Natural fluorescence of normal and neoplastic human colon: a comphrensive "ex vivo" study. Lasers Surgery Medicine, 16: 48-60.
  12. Bottiroli G., Croce A.C., Locatelli D., Nano R., Giombelli E., Messina A., Benericetti E. 1998. Brain tissue autofluorescence: an aid for intraoperative delineation of tumor resection margins. Cancer Detect. Prev., 22: 330-339.
  13. Bouhassira, E., Westerman, K. & Leboulch, P. (1997). Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange. Blood 90, 3332-3344.
  14. Breimer ME, Svalander CT, Haraldsson B, Björck S. (1996)Physiological and histological characterisation of a pig kidney in vitro perfusion model for xenotransplantation studies. Scand J Urol Nephrol.; 30: 213-221.
  15. Brophy B, Smolenski G, Wheeler T, Wells D, L'Huillier P & Laible G (2003) Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein. Nat Biotechnol 21, 157-62.
  16. Brunetti D, Perota A, Lagutina I, Colleoni S, Duchi R, Calabrese F, Seveso M, Cozzi E, Lazzari G, Lucchini F, Galli C. Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Pigs Derived from In Vitro Transfected Adult Fibroblasts. Cloning Stem Cells 2008.
  17. Cancio, R., A. Mai, D. Rotili, M. Artico, G. Sbardella, I. Clotet-Codina, J. A. Este, E. Crespan, S. Zanoli, U. Hubscher, S. Spadari, and G. Maga. (2007). Slow-, tight-binding HIV-1 reverse transcriptase non-nucleoside inhibitors highly active against drug-resistant mutants. ChemMedChem 2:445-8.
  18. Carey, B. et al. (2009).Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A 106, 157-162
  19. Casiraghi F, Azzollini N, Cassis P, Imberti B, Morigi M, Cugini D, Cavinato RA, Todeschini M, Solini S, Sonzogni A, Perico N, Remuzzi G, Noris M. (2008)Pretransplant infusion of mesenchymal stem cells prolongs the survival of a semiallogeneic heart transplant through the generation of regulatory T cells. J Immunol.;181: 3933-3946.
  20. Cereda,C., Cova E., Dipoto C., Mazzini G., Corato M., Ceroni M. (2006). Effect of nitric oxide on lymphocytes from sporadic amyotrophic lateral sclerosis patients:toxic or protective role? Neurol. Sci., 27: 312-316.
  21. Chinnasamy, D. et al. (2006).Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI. Virol J 3, 14
  22. Cova E., Cereda C., Galli A., Curti D., Finotti C., Di Poto C., Corato M., Mazzini G., Ceroni M. (2006). Modified expression of Bcl-2 and SOD1 proteins in lymphocytes from sporadic ALS patients. Neurosci. Lett., 399: 186-190.
  23. Cozzi E, Bosio E, Seveso M, Vadori M & Ancona E (2005) Xenotransplantation-current status and future perspectives. Br Med Bull 75-76, 99-114.
  24. Crespi F., Croce Ac., Fiorani S., Masala B., Heidbreder C., Bottiroli G. (2004). Autofluorescence spectrofluorometry of central nervous system (CNS) neuromediators. Lasers Surgery and Medicine, 34: 39-47.
  25. Croce A.C., Bottiroli G., Roda E., Pisu M.B., Bernocchi G. (2006). Autofluorescence and structural-molecular changes of the developing normal and injured rat cerebellum cortex. Lasers Surgery Medicine, 38: 598-607.
  26. Croce A.C., De Simone U., Vairetti M, Ferrigno A.,Bottiroli G. (2007). Autofluorescence properties of rat liver under hypermetabolic conditions. Photochem. Photobiol. Sci., 6: 1202-1209.
  27. Croce A.C., Spano A., Locatelli D., Barni S., Sciola L., Bottiroli G. (1999). Dependence of fibroblast autofluorescence properties on normal and transformed conditions. Role of the metabolic activity. Photochem. Photobiol., 69: 364-375.
  28. Croce Ac., De Simone U., Vairetti M., Ferrigno A., Boncompagni E., Freitas I., Bottiroli G. (2008). Liver autofluorescence properties in animal model under altered nutritional conditions. Photochem. Photobiol. Sci., 7: 1046-1053.
  29. Croce Ac., Ferrigno A., Vairetti M., Bertone R., Freitas I.,Bottiroli G. (2004). Autofluorescence properties of isolated rat hepatocytes under different metabolic conditions. Photochem. Photobiol. Sci., 3: 920-926.
  30. Croce Ac., Ferrigno A., Vairetti M., Bertone R., Freitas I.,Bottiroli G. (2005). Autofluorescence spectroscopy of rat liver during experimental transplantation procedure. An approach for hepatic metabolism assessment. Photochem. Photobiol. Sci., 4: 583-590.
  31. Croce Ac., Fiorani S., Locatelli D., Nano R., Ceroni M., Tancioni F., Giombelli E., Benericetti E., Bottiroli G. (2003). Diagnostic potential of Autofluorescence for an assisted intraoperative delineation of glioblastoma resection margins. Photochem. Photobiol., 77: 309-318.
  32. D'Apice, A. & Cowan, P. (2008). Gene-modified pigs. Xenotransplantation 15, 87-90.
  33. Della Porta M.G., Malcovati L., Rigolin G.M., Rosti V., Bonetti E., Travaglino E., Boveri E., Gall A., Boggi S., Ciccone M., Pramparo T., Mazzini G., Invernizzi R., Lazzarino M., Cazzola M. (2008). Immunophenotypic, cytogenetic and functional characterization of circulating endotelial cells. Leukemia, 22: 530-537.
  34. David H. Sachs, Cesare Galli "Genetic manipulation in pigs" Current Opinion in Organ Transplantation 2009, 14:148–153.
  35. Dionigi P., Ferrari A., Jemos V., Luinetti O., Vai L., Guizzetti M., Ferrari C., Jemos K., Spada M., Mazzini G. (2004). Tumor cell proliferation in early gastric cancer: biological and clinical behavior. Hepatogastroenterology, 51: 264-268.
  36. Donnelly, M. et al. (2001).Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'. J Gen Virol 82, 1013-1025
  37. Donnelly, M. et al. (2001).The 'cleavage' activities of foot-and-mouth disease virus 2A sitedirected mutants and naturally occurring '2A-like' sequences. J Gen Virol 82, 1027-1041
  38. Dreassi, E., A. T. Zizzari, F. Falchi, S. Schenone, A. Santucci, G. Maga, and M. Botta. (2009). Determination of permeability and lipophilicity of pyrazolo-pyrimidine tyrosine kinase inhibitors and correlation with biological data. Eur J Med Chem 44:3712-7.
  39. Dufrane D & Gianello P (2008) Pig islet xenotransplantation into non-human primate model. Transplantation 86, 753-60.
  40. Elbashir, S. M., W. Lendeckel, and T. Tuschl. (2001). RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes & development 15:188-200.
  41. Falaschi, A., Abdurashidova, G., Sandoval, O., Radulescu, S., Biamonti, G., and Riva, S. (2007). Molecular and structural transactions at human DNA replication origins. Cell Cycle 6, 1705-1712.
  42. Falchi, F., F. Manetti, F. Carraro, A. Naldini, G. Maga, E. Crespan, S. Schenone, O. Bruno, C. Brullo, and M. Botta. (2009). 3D QSAR models built on structure-based alignments of Abl tyrosine kinase inhibitors. ChemMedChem 4:976-87.
  43. Fang, J. et al. Stable antibody expression at therapeutic levels using the 2A peptide. Nat Biotechnol 23, 584-590 (2005).
  44. Fahrenkrug S. C. , A. Blake, D. F. Carlson, T. Doran, A. Van Eenennaam, D. Faber, C. Galli, Q. Gao, P. B. Hackett, N. Li, E. A. Maga, W. M. Muir, J. D. Murray, D. Shi, R. Stotish, E. Sullivan, J. F. Taylor, M. Walton, M. Wheeler, B. Whitelaw and B. P. Glenn "Precision genetics for complex objectives in animal agriculture" J Anim Sci 2010.88:2530-2539. doi: 10.2527/jas.2010-2847 originally published online Mar 12, 2010;
  45. Feng, Y. Q., Seibler, J., Alami, R., Eisen, A., Westerman, K. A., Leboulch, P., Fiering, S., and Bouhassira, E. E. (1999). Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange. J Mol Biol 292, 779-785.
  46. Friedrich, G. & Soriano, P. (1991).Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5, 1513-1523
  47. Fukushige, S. & Sauer, B. (1992).Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc Natl Acad Sci U S A 89, 7905-7909
  48. Gallo, P., Grimaldi, S., Latronico, M. V., Bonci, D., Pagliuca, A., Gallo, P., Ausoni, S., Peschle, C. and Condorelli, G. (2008). A lentiviral vector with a short troponin-I promoter for tracking cardiomyocyte differentiation of human embryonic stem cells. Gene Ther 15:161-70.
  49. Galli C, Perota A, Brunetti D, Lagutina I, Lazzari G, Lucchini F. "Genetic engineering including superseding microinjection: new ways to make GM pigs". Xenotransplantation 2010; 17: 397–410.  2010 John Wiley & Sons A/S.
  50. Gavrilescu M & Chisti Y (2005) Biotechnology-a sustainable alternative for chemical industry. Biotechnol Adv 23, 471-99.
  51. Gianello P & Dufrane D (2007) [Correction of a diabetes mellitus type 1 on primate with encapsulated islet of pig pancreatic transplant]. Bull Mem Acad R Med Belg 162, 439-49; discussion 449-50.
  52. Hartley, J., Temple, G. & Brasch, M. (2000).DNA cloning using in vitro site-specific recombination. Genome Res 10, 1788-1795.
  53. Hu, T., Fu, Q., Chen, P., Zhang, K. & Guo, D. (2009).Generation of a stable mammalian cell line for simultaneous expression of multiple genes by using 2A peptide-based lentiviral vector. Biotechnol Lett 31, 353-359.
  54. Ibrahimi, A. et al. (2009).Highly Efficient Multicistronic Lentiviral Vectors with Peptide 2A Sequences. Hum Gene Ther.
  55. Irion, S. et al. (2007).Identification and targeting of the ROSA26 locus in human embryonic stem cells. Nat Biotechnol 25, 1477-1482.
  56. Jaenisch R, Young R. (2008) Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell. 132:567-82.
  57. Kaji, K. et al. (2009).Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458, 771-775
  58. Kiefer C.R., Mckenney J.B., Trainor J.F., Lambrecht R.W., Bonkovsky H.L., Lifshitz L.M., Valeri C.R., Snyder L.M. (1998). Porphyrin loading of lipofuscin granules in inflamed striated muscle. Am J Pathol., 153, 703-870.
  59. Kuroiwa Y, Kasinathan P, et al. (2002) Cloned transchromosomic calves producing human immunoglobulin. Nat Biotechnol 20, 889-94.
  60. Kuroiwa Y, Kasinathan P, Matsushita H, Sathiyaselan J, Sullivan EJ, Kakitani M, Tomizuka K, Ishida I & Robl JM(2004) Sequential targeting of the genes encoding immunoglobulin-mu and prion protein in cattle. Nat Genet 36, 775-80.
  61. Laflamme, M. A., Chen, K. Y., Naumova, A. V., Muskheli, V., Fugate, J. A., Dupras, S. K., Reinecke, H., Xu, C., Hassanipour, M., Police, S. et al. (2007). Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol 25:1015-24.
  62. Lai L, Kang JX, et al. (2006) Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nat Biotechnol 24, 435-6.
  63. Lanni C., Racchi M., Mazzini G., Ranzenigo A., Polotti R., Sinforiani E., Olivari L., Barcikowska M., Styczynska M., Kuznicki J., Szybinska., Govoni S., Memo M., Uberti D. (2008) a. Conformationally altered p53: a novel Alzheimer's disease marker? Mol. Psychiatry, 5: 209-211.
  64. Lanni C., Racchi M., Uberti D., Mazzini G., Stanga S., Sinforiani E., Memo M., Govoni S. (2008) b. Pharmacogenetics and pharmagenomics, trends in normal and pathological aging studies: focus on p53. Curr. Pharm. Des., 14: 2665-2671.
  65. Lapinski R, Perico N, Remuzzi A, Sangalli F, Benigni A, Remuzzi G. Angiotensin II modulates glomerular capillary permselectivity in rat isolated perfused kidney. J Am Soc Nephrol. (1996); 7: 653-660.
  66. Lawson JH, Platt JL. Molecular barriers to xenotransplantation. Transplantation. (1996); 62: 303- 310.
  67. Leschik, J., Stefanovic, S., Brinon, B. and Puceat, M. (2008). Cardiac commitment of primate embryonic stem cells. Nat Protoc 3:1381-7.
  68. Lex M (2008) Industrial biotechnology takes off in Europe. Sci Prog 91, 39-64.
  69. Li G, Huang J, Jiang P, Li Y, Jiang W & Wang X 2007 Suppression of porcine reproductive and respiratory syndrome virus replication in MARC-145 cells by shRNA targeting ORF1 region. Virus Genes 35, 673-9.
  70. Locatelli, G. A., G. Gosselin, S. Spadari, and G. Maga. (2001). Hepatitis C virus NS3 NTPase/helicase: different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting step. J Mol Biol 313:683-94.
  71. Locatelli, G. A., S. Spadari, and G. Maga. (2002). Hepatitis C virus NS3 ATPase/helicase: an ATP switch regulates the cooperativity among the different substrate binding sites. Biochemistry 41:10332-42.
  72. Maga EA 2005 Genetically engineered livestock: closer than we think? Trends Biotechnol 23, 533- 5.
  73. Maga, G., M. Amacker, N. Ruel, U. Hubscher, and S. Spadari. (1997). Resistance to nevirapine of HIV-1 reverse transcriptase mutants: loss of stabilizing interactions and thermodynamic or steric barriers are induced by different single amino acid substitutions. J Mol Biol 274:738-47.
  74. Maga, G., M. Radi, S. Zanoli, F. Manetti, R. Cancio, U. Hubscher, S. Spadari, C. Falciani, M. Terrazas, J. Vilarrasa, and M. Botta. (2007). Discovery of non-nucleoside inhibitors of HIV-reverse transcriptase competing with the nucleotide substrate. Angew Chem Int Ed Engl 46:1810-3.
  75. Maga, G., S. Gemma, C. Fattorusso, G. A. Locatelli, S. Butini, M. Persico, G. Kukreja, M. P. Romano, L. Chiasserini, L. Savini, E. Novellino, V. Nacci, S. Spadari, and G. Campiani. (2005). Specific targeting of hepatitis C virus NS3 RNA helicase. Discovery of the potent and selective competitive nucleotide-mimicking inhibitor QU663. Biochemistry 44:9637-44.
  76. Magnani, E., Bartling, L. & Hake, S. From Gateway to MultiSite Gateway in one recombination event. BMC Mol Biol 7, 46 (2006). Manfroni, G., J. Paeshuyse, S. Massari, S. Zanoli, B. Gatto, G. Maga, O. Tabarrini, V.
  77. Cecchetti, A. Fravolini, and J. Neyts. (2009). Inhibition of subgenomic hepatitis C virus RNA replication by acridone derivatives: identification of an NS3 helicase inhibitor. J Med Chem 52:3354-65.
  78. Marchesini R., Pignoli E., Tomatis S., Fumagalli S., Sichirollo A.E., Di Palma S., Dal Fante M., Spinelli P., Croce A.C., Bottiroli G. (1994). Ex vivo optical properties of human colon tissue. Lasers Surgery and Medicine, 15: 351-357.
  79. Matsunari, H., and Nagashima, H. (2009). Application of genetically modified and cloned pigs in translational research. J Reprod Dev 55, 225-230.
  80. Mazzini G., Ferrari C., Baraldo N., Mazzini M., Angelini M. (2004). Improvements in fluorescence microscopy allowed by high power light emitting diodes. FORMATEX. Current Issues on Multidisciplinary Microscopy Research and Education,A. Mendez-Vilas, L.Labajos- Broncano, Badajoz, Spain.
  81. Mazzini G., Ferrari C., Erba E. (2003). Dual excitation multi-fluorescence flow cytometry for detailed analyses of viability and apoptotic cell transition. Eur J Histochem., 47: 289-298.
  82. McManus RP, O'Hair DP, Beitzinger JM, Schweiger J, Siegel R, Breen TJ & Olinger GN (1993) Patients who die awaiting heart transplantation. J Heart Lung Transplant 12, 159-71; discussion 172.
  83. Monici M., Pratesi R., Bernabei P.A., Caporale R., Croce A.C., Bottiroli G. (1995). Natural fluorescence of white blood cells: spectroscopic and imaging study. J. Photochem. Photobiol., 30: 29 - 37.
  84. Morgan D.C., Wilson J.E., Macaulay C.E., Mackinnon N.B., Kenyon J.A., Gerla P.S., Dong C., Zeng H., Whitehea.D., Thompson C.R., Mcmanus B.M. (1999). New method for detection of heart allograft rejection: validation of sensitivity and reliability in a rat heterotopic allograft model. Circulation, 100: 1236-1241.
  85. Nishida K, Yamaguchi O, Otsu K. (2008). Crosstalk between autophagy and apoptosis in heart disease. Circ Res., 15: 343-351.
  86. Niwa, H., Yamamura, K. & Miyazaki, J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193-199 (1991).
  87. Noris M, Casiraghi F, Todeschini M, Cravedi P, Cugini D, Monteferrante G, Aiello S, Cassis L, Gotti E, Gaspari F, Cattaneo D, Perico N, Remuzzi G. (2007)Regulatory T cells and T cell depletion: role of immunosuppressive drugs. J Am Soc Nephrol.; 18: 1007-1018.
  88. Noris M, Cugini D, Casiraghi F, Azzollini N, De Deus Viera Moraes L, Mister M, Pezzotta A, Cavinato RA, Aiello S, Perico N, Remuzzi G. (2001)Thymic microchimerism correlates with the outcome of tolerance-inducing protocols for solid organ transplantation. J Am Soc Nephrol.; 12: 2815-2826.
  89. Nyabi, O. et al. (2009).Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells. Nucleic Acids Res 37, e55
  90. Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y. (1997). 'Green mice' as a source of ubiquitous green cells. FEBS Lett 407, 313-319
  91. Paixao, S., Colaluca, I. N., Cubells, M., Peverali, F. A., Destro, A., Giadrossi, S., Giacca, M., Falaschi, A., Riva, S., and Biamonti, G. (2004). Modular structure of the human lamin B2 replicator. Mol Cell Biol 24, 2958-2967.
  92. Pelosi, E., Valtieri, M., Coppola, S., Botta, R., Gabbianelli, M., Lulli, V., Marziali, G., Masella, B., Müller, R., Sgadari, C., Testa, U., Bonanno, G., Peschle, C. (2002). Identification of the hemangioblast in postnatal life. Blood. 100:3203-8
  93. Perehinec, T. et al. (2007).Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria. BMC Mol Biol 8, 80
  94. Perico N, Benigni A, Gabanelli M, Piccinelli A, Ròg M, De Riva C, Remuzzi G. (1992)Atrial natriuretic peptide and prostacyclin synergistically mediate hyperfiltration and hyperperfusion of diabetic rats. Diabetes.; 41: 533-538.
  95. Perico N, Cornejo RP, Benigni A, Malanchini B, Ladny JR, Remuzzi G. (1991)Endothelin induces diuresis and natriuresis in the rat by acting on proximal tubular cells through a mechanism mediated by lipoxygenase products. J Am Soc Nephrol.; 2: 57-69.
  96. Perico N, Dadan J, Gabanelli M, Remuzzi G. (1990)Cyclooxygenase products and atrial natriuretic peptide modulate renal response to endothelin. J Pharmacol Exp Ther.; 252: 1213-1220.
  97. Perico N, Delaini F, Lupini C, Benigni A, Galbusera M, Boccardo P, Remuzzi G. (1989)Blunted excretory response to atrial natriuretic peptide in experimental nephrosis. Kidney Int.; 36: 57-64.
  98. Perico N, Remuzzi G. (1997)Xenotransplantation: problems and prospects. Nephrol Dial Transplant.; 12 (Suppl 1): 59-64.
  99. Perico N, Zoja C, Corna D, Rottoli D, Gaspari F, Haskell L, Remuzzi G. V (2009) Vasopressin receptor antagonism potentiates the renoprotection of renin-angiotensin system inhibition in rats with renal mass reduction. Kidney Int. Jul 22. [Epub ahead of print]
  100. Poulsen EU, Frøkiaer J, Jørgensen TM, Pedersen EB, Rehling M. (1997)Renal functional reserve in pigs: renal haemodynamics, renal tubular function and salt and water homeostatic hormones during amino acid and dopamine stimulation. Clin Physiol.; 17: 57-69.
  101. Radi, M., C. Falciani, L. Contemori, E. Petricci, G. Maga, A. Samuele, S. Zanoli, M. Terrazas, M. Castria, A. Togninelli, J. A. Este, I. Clotet-Codina, M. Armand-Ugon, and M. Botta. (2008). A multidisciplinary approach for the identification of novel HIV-1 non-nucleoside reverse transcriptase inhibitors: S-DABOCs and DAVPs. ChemMedChem 3:573-93.
  102. Radi, M., G. Maga, M. Alongi, L. Angeli, A. Samuele, S. Zanoli, L. Bellucci, A. Tafi, G. Casaluce, G. Giorgi, M. Armand-Ugon, E. Gonzalez, J. A. Este, M. Baltzinger, G. Bec, P. Dumas, E. Ennifar, and M. Botta. (2009). Discovery of chiral cyclopropyl dihydro-alkylthiobenzyl- oxopyrimidine (S-DABO) derivatives as potent HIV-1 reverse transcriptase inhibitors with high activity against clinically relevant mutants. J Med Chem 52:840-51.
  103. Radi, M., L. Angeli, L. Franchi, L. Contemori, G. Maga, A. Samuele, S. Zanoli, M. Armand- Ugon, E. Gonzalez, A. Llano, J. A. Este, and M. Botta. (2008). Towards novel S-DABOC inhibitors: Synthesis, biological investigation, and molecular modeling studies. Bioorg Med Chem Lett.
  104. Ramunujam N. (2000) a. Fluorescence spectroscopy in vivo. Encyclopedia of Analytical Chemistry, 20-56, John Wiley.
  105. Ramunujam N. (2000) b. Fluorescence spectroscopy of neoplastic and non-neoplastic tissues, Neoplasia, 2: 89-117.
  106. Reddy, A. M., Y. Zheng, G. Jagadeeswaran, S. L. Macmil, W. B. Graham, B. A. Roe, U. Desilva, W. Zhang, and R. Sunkar. (2009). Cloning, characterization and expression analysi of porcine microRNAs. BMC Genomics 10:65.
  107. Remuzzi G, Perico N, Benigni A. (2002) New therapeutics that antagonize endothelin: promises and frustrations. Nat Rev Drug Discov.; 1: 986-1001
  108. Richards-Kortum R. (1996). Quantitative optical spectroscopy for tissue diagnosis. Annu. Rev. Phys. Chem., 47: 555-606.
  109. Rideout WM 3rd, Hochedlinger K, Kyba M, Daley GQ, Jaenisch R. (2002) Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell. 109:17-27.
  110. Rogers CS, Hao Y, et al. (2008) Production of CFTR-null and CFTR-DeltaF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer. J Clin Invest 118, 1571-7.
  111. Rogers CS, Stoltz DA, et al. (2008) Disruption of the CFTR gene produces a model of cystic fibrosis in newborn pigs. Science 321, 1837-41.
  112. Ryan, M. & Drew, J. (1994). Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J 13, 928-933
  113. Sandrin MS, Vaughan HA, Dabkowski PL, McKenzie IF. (1993) Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes. Proc Natl Acad Sci U S A.; 90: 11391-11395.
  114. Sauer, B. & Henderson, N. (1988)Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc Natl Acad Sci U S A 85, 5166-5170.
  115. Sgreccia E, Calipari M & Lavitrano M (2001) Church backing depends on ethical use of animals. Nature 414, 687.
  116. Shao, L. et al. (2009).Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res 19, 296-306
  117. Soriano, P. (1999).Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21, 70-71
  118. Stram, Y. and L. Kuzntzova. (2006). Inhibition of viruses by RNA interference. Virus genes 32:299-306
  119. Szymczak, A. et al. (2004).Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22, 589-594
  120. Thomas, K. & Capecchi, M. (1987) Site-directed mutagenesis by gene targeting in mouse embryoderived stem cells. Cell 51, 503-512.
  121. Tomasoni S, Azzollini N, Casiraghi F, Capogrossi MC, Remuzzi G, Benigni A. (2000) CTLA4Ig gene transfer prolongs survival and induces donor-specific tolerance in a rat renal allograft. J Am Soc Nephrol.; 11: 747-752.
  122. Trichas, G., Begbie, J. & Srinivas, S. (2008)Use of the viral 2A peptide for bicistronic expression in transgenic mice. BMC Biol 6, 40.
  123. Vintersten, K. et al. (2004). Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals. Genesis 40, 241-246
  124. Vodicka P, Smetana K, Jr., Dvorankova B, Emerick T, Xu YZ, Ourednik J, Ourednik V & Motlik J (2005) The miniature pig as an animal model in biomedical research. Ann N Y Acad Sci 1049, 161-71.
  125. Wall RJ, Powell AM, Paape MJ, Kerr DE, Bannerman DD, Pursel VG, Wells KD, Talbot N & Hawk HW (2005) Genetically enhanced cows resist intramammary Staphylococcus aureus infection. Nat Biotechnol 23, 445-51.
  126. Wang T.D. & Van Dam J. (2004). Optical biopsy: a new frontier in endoscopic detection and diagnosis. Clin. Gastroenterol. Hepatol., 2: 744-753.
  127. Wirth, D., Gama-Norton, L., Riemer, P., Sandhu, U., Schucht, R., and Hauser, H. (2007). Road to precision: recombinase-based targeting technologies for genome engineering. Curr Opin Biotechnol 18, 411-419.
  128. Woltjen, K. et al. (2009).piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766-770
  129. Yamada K, Yazawa K, et al. (2005) Marked prolongation of porcine renal xenograft survival in baboons through the use of alpha1,3-galactosyltransferase gene-knockout donors and the cotransplantation of vascularized thymic tissue. Nat Med 11, 32-4.
  130. Yang, L., Soonpaa, M. H., Adler, E. D., Roepke, T. K., Kattman, S. J., Kennedy, M., Henckaerts, E., Bonham, K., Abbott, G. W., Linden, R. M. et al. (2008). Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 453:524-8.
  131. Zanarini, S., Della Ciana, L. Marcaccio, M., Marzocchi, E. Paolucci, F., Prodi L. (2008). Electrochemistry and Electrochemiluminescence of Ru(II)-tris(bathophenanthroline-disulfonate)]4- in Aprotic Conditions and Aqueous Buffers, J. Phys. Chem. B, 112: 10188-10193.
  132. Zanoli, S., S. Gemma, S. Butini, M. Brindisi, B. P. Joshi, G. Campiani, C. Fattorusso, M. Persico, E. Crespan, R. Cancio, S. Spadari, U. Hubscher, and G. Maga. (2008). Selective targeting of the HIV-1 reverse transcriptase catalytic complex through interaction with the "primer grip" region by pyrrolobenzoxazepinone non-nucleoside inhibitors correlates with increased activity towards drug-resistant mutants. Biochem Pharmacol 76:156-68.